42 SFF file (21 Runs) Assembly with Modified Newbler available HERE.
32 SFF file (16 Runs) Assembly with the contig names in the PPT below is HERE
There are no Paired End reads in this assembly. “So “Scaffold” refers to the activation of the heterozygote mode collapsing algorithm (and the output of the “scaffolds” only include contigs larger than 2kb, instead of the large contigs which are larger than 500 bp).”- James Knight
508Mb file. Must use Firefox to download with a Mac. Safari doesnt work.
JaneOme has DATA ACCESS for 5 more strains- Raw data only as of 4/20/2012
Amino Acid Sequences of the THCA Synthase Sequences.THCAS-Like 3 is differentially expressed in the Roots as opposed to the Flowers and has a unique N-Terminal peptide. LA = LA Confidential. PK = Purple Kush. CD = ChemDawg.Reference THCA Synthase published by Kojoma et al.
Multiple Alignments for like contigs amongst the strains shows subtle amino acid differences. LA Confidential THCA Synthase Like 3 aligned to Purple Kush THCA-3 like gene.
Zhang, X., Feschotte, C., Zhang, Q., Jiang, N., Eggleston, W.B., and Wessler, S.R. 2001. P instability factor: an active maize transposon system associated with the amplification of Tourist-like MITEs and a new superfamily of transposases. Proc Natl Acad Sci U S A 98(22): 12572-12577.
Annotation of the THCA synthase gene with FAD binding domains and mPIF integration signals.
Interesting Findings- THCA Synthase may have a P-Instability Factor internal to the gene sequence.
Potential mechanism for cannabinoid synthase replication via P-Instability Factor.
AGBT 2012 Roche Presentation
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- Potential mechanism for cannabinoid synthase replication via P-Instability Factor.
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- Multiple Alignments for like contigs amongst the strains shows subtle amino acid differences
