2 Lanes of ILMN assembled with CLC on C.Sativa. This is without Scaffolding. We are working with CLC to test their latest assemblers.
Insert size came back at 238bp but was set at 250-600. Stringency set at 0.9.
- Prep7_1-3_1_sequence (paired) summary report-2
- Prep7_1-1_1_Orig_Assembly_Stats (paired) summary report
- Prep7_1-7_1_Lanes7_8_sequence (paired) de novo assembly report
BLAST REPORTS OF ALL 174K CONTIGS AGAINST THC SYNTHASE
- Prep7_1-3_1_sequence(paired)contig18025 BLAST
- Prep7_1-3_1_sequence(paired)contig19853 BLAST
- Prep7_1-3_1_sequence(paired)contig28261 BLAST
- Prep7_1-3_1_sequence(paired)contig31598 BLAST
- Prep7_1-3_1_sequence(paired)contig32061 BLAST
- Prep7_1-3_1_sequence(paired)contig38887 BLAST
- Prep7_1-3_1_sequence(paired)contig54345 BLAST
- Prep7_1-3_1_sequence(paired)contig69418 BLAST
- Prep7_1-3_1_sequence(paired)contig70172 BLAST
- Prep7_1-3_1_sequence(paired)contig73347 BLAST
- Prep7_1-3_1_sequence(paired)contig87013 BLAST
- Prep7_1-3_1_sequence(paired)contig108128 BLAST
- Prep7_1-3_1_sequence(paired)contig137854 BLAST
- Prep7_1-3_1_sequence(paired)contig165876 BLAST
Assembly reports for 454 Assemblies with 20 SFF files
- LA-454_Assem10_0.8_20FSFF_(single) summary report
- Assem_11_20SFF_0.9_Vote_summary report
- LA-454_Assem_12- summary report
- LA-454_Assem_13_32SFF_0.99_remap(single) summary report
PCR and Sanger Sequencing show high degree of polymorphism in the THC synthase genes. Assembly implies these regions are easily non specifically PCR’d and may create artifacts.
