Using the most advanced DNA sequencing tools, billions of DNA molecules can be sequenced in parallel for comprehensive genetic classification of Cannabis Strains. Medicinal Genomics has sequenced 65,000X more Cannabis DNA Sequence than exists in the public Databases in 2011.
We have used HiSeq 2×100 sequencing for 131Gb of data on Chemdawg.
We also have access to 750bp 454 Sequencing for over 10Gb of Sequence on triple backcrossed, Cannabis Cup winner LA Confidential. A strain also well known for its use in extreme cases of Autism: http://uf4a.org/.
This sequencing technology has aided in phasing many CNVs in the genome governing chemotype.
454 offers the longest massively Parallel sequencing which enables phasing of highly polymorphic regions in CNVs of synthase genes which govern Chemotype
In addition, care has been taken to sequence Cannabis strains which have strict quality control and cloning efforts under way with DNA Genetics, NL.
Strains of interest Include:
- Triple Backcrossed Pure Indica – LA Confidential.
- Sativa, Hybrid- ChemDawg- Amsterdam
- Recon- DNA Genetics cross with LA Confidential and Cannadential (offspring of female LA Confidential and Cannalope).
- Chocolope- DNA Genetics
Massively Parallel short read sequencers like SOLiD and ILMN help to provide affordable high coverage of Cannabis genomes once there is a good reference genome.