In celebration of Colorado and Washington’s move to legalize cannabis we are releasing a 5X draft sequence of another cultivar called Dr. Grinspoon.
This technique utilized 20ng of DNA and in theory could be performed non-destructively on a seed such that one could sequence the genome of a seed and then choose to plant or not plant it. The DNA isolation and Library construction techniques each cost under $10 respectively. The Sequencing cost ~$1000 but had this been performed on your own non-outsourced HiSEQ it would have likely cost $200.
CLCbio De Novo Assembly (Draft ..only 5X but will likely be 50X of chloroplast and mito genomes)
Thank you to all Jane-Ome subscribers. This work was funded by your curiosity!
This is the first decoded genome funded purely through an education iPad App!
Our Position on Gene Patents:
We fundamentally do not believe DNA sequences should be the subject matter of patents. Despite this there are over 4700 gene patents filed on the human genome and articles written by Christopher Mason showcase how many human genomes are covered by gene patents and how these gene patents are often poorly written and non-specific. Chris_Mason_Table_S1. Jensen & Murray also highlight this in the below articles.
We side with Stephan Kinsella on Intellectual Property being oxymoronic. Property rights cover property. Property must
2)Not be infinately replicable
Ideas are neither of these.
A reading of these papers will show that there are on average 5 patents per human gene! This implies the USPTO is not very effective at prior art searches and our efforts to put the genome public on the Amazon cloud in August of 2011 may not prevent the cannabis genome from becoming the entangled patent thicket we experience on the human genome. For this reason we have filed the below files with the USPTO so they cannot miss them. They are registered with their database and should appear in any preliminary search report. This is important as the human race will produce more data in the next 6 months than in its entire existence and this ‘Kurzweilian‘ like accelerated growth is becoming ever more untenible for the USPTO to adequately search.
This has created considerable additional expense for us (over $20,000). This patent application will not issue. Once published it will be abandoned. Once abandonded they will act as prior art to any future attempts at patenting the cannabis genome and it will leave the vast majority of the cannabis genome Public forever! This is an extraordinary act of charity on behalf of often mis-represented “greedy/profit driven” company considering over $300K has been put into ensuring this genome will remain open source for all to study. We may not get this investment back in direct profits but we believe this rising tide can float all boats in an ever important market for human health and well being. This should enable product development with the information far less complicated than sequencing human genes. Enjoy!