Speed, Confidentiality, and Precision

With the youPCR™ Point-of-Grow Genetic Testing Platform cannabis growers can quickly, easily, and inexpensively screen cannabis plants for genetic markers on-site, without involving a third-party testing lab. The colorimetric assay provides a simple yes/no answer about whether a cannabis plant contains a specific DNA marker. If the liquid changes color, the marker is present.

Fast

Performing marker-selected screening at the point-of-grow means you get results in hours, not days.

Confidential

Keep your marker selection efforts in-house, and stop shipping cannabis material to other labs that may intend to compete with you with your own data.

Easy

The youPCR process requires no advanced laboratory techniques or expensive equipment.

youPCR Tests

youPCR Gender Detection Test

Growing from seeds? The youPCR Gender Detection Test looks for the Y chromosome in the plant’s DNA, so you can quickly and accurately identify male cannabis plants in your grow. Over 95% accurate.

youPCR Powdery Mildew

Powdery Mildew is the most destructive fungal pathogen for Cannabis. It is an obligate biotroph that can vascularize into the plant tissue and remain invisible to a grower. With youPCR, growers can screen for powdery mildew and remove infected plants before they infect others.

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youPCR CBD Marker Test

Breeding high-CBD strains? The youPCR CBD Marker Test looks for the Bd allele in the plant’s DNA, which is associated with CBD synthesis.

Learn More

The youPCR Process

1. Collect 4mm punches from each plant leaf into a strip tube.

2. Add youPCR Solution A to each well.

3. Heat the wells with miniPCR machine or thermocycler for 25 minutes.

4. Remove liquid from each well and pipette into a new strip tube that contains youPCR Solution B reagents and the Colorimetric LAMP assay.

5. Heat the new strip tube for another 50 mins.

6. See the results! Samples that contain the target marker will change from pink to yellow, while negative results will remain pink.

Ready to Get Started?

Step 1: Purchase Chemical Reagents

The chemical reagents needed to use the youPCR Point-of-Grow Genetic Testing Platform are available for pre-order on the Medicinal Genomics webstore. They will ship in May 2017.

To run the youPCR Gender Detection Test you will need:

To run the youPCR CBD Detection Test, you will need:

Step 2: Select a Thermocylcer

The youPCR platform can be run on a miniPCR thermocycler or standard-sized thermocyclers. Customers should consider volume when choosing which to use with the youPCR platform.

MiniPCR Thermocycler

(Test 6 samples per instrument run)

Purchase from miniPCR (Contact Medicinal Genomics to acquire a Discount Coupon Code on this device)

Standard Thermocycler

(Test 94 samples per instrument run)

Any programmable 96-well thermalcycler with a heated lid will work. qPCR detection is not required.

Step 3: Purchase Ancillary Equipment and Consumables

 

If you do not have an in-house laboratory, you will need to purchase some additional equipment in order to complete the youPCR protocol. The equipment needed will depend on which thermocycler you chose in step 2. Click the links below to view the list of materials.

View miniPCR Thermocycler Equipment list

Hardware

Consumables and ancillary supplies

Optional but recommended supplies

  • Eppendorf tube rack (USA Scientific, #2380-1008 or similar)
  • For more accurate pipetting we recommend a P10 single channel micropipette, Purchase from miniPCR
  • Table/lab bench covering (VWR, #89126-794 or similar), Purchase from VWR
View Standard Thermocycler Equipment list

Hardware