Australian Bastard Hybrid | Medicinal Genomics

This cultivar is not the original Australian Bastard but a Hybrid with a THCA+ plant.
Kannapedia
StrainSEEK data- Forward Reads
StrainSEEK data- Reverse Reads
VCF File Hashed into BTC
ITS Microbiome Reads
OneCodex Analysis
Methods for this work are described below

Australian Bastard now in the blockchain. 035e74ef2698711519c671e2628cd313f29f7e97ece11cd0a01a6d95f1faeb17 https://t.co/9qPgleixSa

— Kevin McKernan (@Kevin_McKernan) November 15, 2015

Image data provided by the WercShop.

Comments from submitter-

“I have to admit, after seeing these plants in person they’re really something else! They’re so deformed you could walk by or through them and not even recognize they’re Cannabis. They exhibit a leaf mutation that instead of producing typical blades/laminas they form these modified leaflets with little serration(and in select individuals variegation). In some of the photos attached you’ll find a size comparison of fan leaves from a drug variety and an ABC. These were sampled from approximately the same location on each plant and to further demonstrate their size difference I placed an ABC hybrid fan leaf onto a fan leaf of an adult drug variety. In one of the last photos you’ll see a small plant no more than 2 ft. tall. According to our source, this phenotype most resembles the plants from the original P1 generation.”

According to John McPartland (pers. commun., 2015)- “Some Cannabis in Australia has a very unique provenance. William Roxburgh, a British botanist stationed in Calcutta, sent germplasm from India to Australia in 1802. More Indian seed was sent in 1803. Colonists grew it in the Singleton district of the Hunter Valley into the 1820s. It no doubt gave rise to a huge patch of feral Cannabis that covered 30 km² (12 mi²) grew along the flood plain of the Hunter River 160 km (100 mi) north of Sydney. The feral hemp was famously psychoactive.

Beginning in the 1860s, imported camels and camel drivers helped explore and settle Australia’s outback. The cameleers were Muslims and Sikhs from present-day Pakistan and Afghanistan. Perhaps 3000 cameleers lived in south-central Australia. They smoked from “narghiles,” and ate “marjoms” (Stevens 1989). The cameleers may have grown Cannabis (Rainford 2009).“

Video of the plants and their hybrids- IMG_3341

Single Molecule Sequencing of THCAS in Asutralian Bastard reveals a single copy THCAS gene with 2130 Primers. For methods see Single molecule sequencing of THCA synthase reveals copy number variation in modern drug-type Cannabis sativa L.

Other InActive Alleles (align with Q33DQ2.1) found with PacBio sequencing of Onofri Primers. Notice the N22S and P81S variants. We have seen this haplogroup in WiFi and C4. Could also been inherited from the THCAS line.

3rd Haplogroup in Australian Bastard aligns to InActive Allele (Q33DQ2.1)