Microbial contamination on cannabis products represents one of the most significant threats to cannabis consumers, particularly immunocompromised patients who could develop harmful infections. 

To protect consumers in their jurisdiction, most regulators in the United States require third-party labs perform microbial safety testing for certain microbes at certain levels. The targets the regulators set can vary widely. For example, California requires labs to test for six specific pathogenic species of Aspergillus (A. flavus, A. fumigatus, A. niger, A. terreus), Salmonella, and Shiga toxin-producing E. coli (STEC). If California labs detect any of those targets in any concentration, the sample fails. On the other hand, Massachusetts regulations set allowable limits for large groups of microorganisms, such as aerobic bacteria, yeast and mold, coliforms, and bile-tolerant gram-negative bacteria. 

The maps below provide a snapshot of the different microbial targets each state requires. The subsequent table provides more detail about each state’s cannabis microbial testing regulations, including specific requirements for different product types, when applicable.

Medicinal Genomics offers a variety of PathoSEEK® Microbial Safety Testing kits that can be used to test any cannabis matrix for any of the targets listed below. Learn more about our Complete Microbial Safety Testing Solution.

Threshold Amounts By State (CFU)

Total Aerobic Count

Aspergillus (unspecified)

Bile-Tolerant Gram-Negative Bacteria

E. coli

Total Enterobacteriaceae

Pseudomonas aeruginosa

Salmonella

Staphylococcus aureus

STEC

Total Yeast and Mold

State Cannabis Microbial Testing Regulations

Last Updated: August 13, 2020

State Target Acceptable Limit
Alabama
View Regulations

.

No Testing Regulations
Alaska
View Regulations

Medical +Recreational
Legalized
Salmonella Less than 1 colony forming unit CFU/g
STEC E.Coli Less than 1 colony forming unit CFU/g
Aspergillus

  • A. fumigatus
  • A. flavus
  • A. niger
Less than 1 colony forming unit CFU/g
Arizona
View Regulations

Medical Legalized
Salmonella Detectable in 1 gram
E.Coli Less than 100 colony forming unit CFU/g
Aspergillus

  • A. fumigatus
  • A. flavus
  • A. niger
  • A. terreus
Detectable in 1 gram (Inhalable Products)
Arkansas
View Regulations

Medical Legalized
Total Coliform If detected, sample must then be tested for E. coli
E.Coli If more then 100 CFU/g sample fails
California
View Regulations

Medical + Recreational
Legalized
Inhalable cannabis goods
Salmonella is not detected in 1 gram
STEC E.Coli is not detected in 1 gram
Aspergillus

  • A. fumigatus
  • A. flavus
  • A. niger
  • A. terreus
is not detected in 1 gram
Non-inhalable cannabis goods
Salmonella is not detected in 1 gram
STEC E.Coli is not detected in 1 gram
Berkley, California
View Regulations

Medical + Recreational Legalized
Salmonella
E.Coli
Total Aerobic
Total Yeast Mold
Pseudomonas
Total Coliform
Colorado
View Regulations

Medical + Recreational Legalized
Regulated Marijuana Flower and trim; Regulated Marijuana Products (other than Audited Product); Water-Based, Heat/Pressure-Based, and Food-Based Medical Marijuana Concentrate; Water-Based, Heat/Pressure-Based, and Food-Based Retail Marijuana Concentrate; Industrial Hemp Products; Pressurized Metered Dose Inhalers; Vaporizer Delivery Device
Salmonella < 1 Colony Forming Unit CFU
STEC E.Coli < 1 Colony Forming Unit CFU
Total Yeast Mold < 104 Colony Forming Unit CFU
Administration by metered dose nasal spray or vaginal administration
Total Yeast Mold ≤101 cfu/ml or ≤101 cfu/g
Total Aerobic ≤102 cfu/ml or ≤102 cfu/g
Staphylococcus Aureus Absent in 1 ml or 1 g
Pseudomonas Aerugnosa Absent in 1 ml or 1 g
Bile Tolerant Gram Negative Bacteria Absent in 1 ml or 1 g
(Only tested for administration by metered dose nasal spray products)
Candida Albicans Absent in 1 ml or 1 g
(Only tested for vaginal administration products)
Rectal administration products
Total Yeast Mold ≤102 cfu/ml or ≤102 cfu/g
Total Aerobic ≤103 cfu/ml or ≤103 cfu/g
Connecticut
View Regulations
USP Regulations

Medical Legalized
Salmonella Not detected in 10g
E.Coli Not detected in 10g
Total Aerobic <105 Colony Forming Unit CFU
Total Yeast Mold <103 Colony Forming Unit CFU
Bile Tolerant Gram Negative Bacteria <103 Colony Forming Unit CFU
Pseudomonas Absense in 1g or 1ml
Delaware
View Regulations

Medical Legalized
E.Coli N/A
Pseudomonas Aeruginosa N/A
Aspergillus spp

  • A. fumigatus
  • A. flavus
  • A. niger
  • A. terreus
N/A
Total Aerobic N/A
Total Yeast Mold N/A
Total Coliform N/A
Staphlococcus Aureus N/A
District of Columbia
View Regulations

Medical + Recreational
Legalized
There is no testing regulations
Florida
View Regulations

Medical Legalized
Salmonella <1 CFU per gram
STEC E.Coli <1 CFU per gram
Aspergillus spp

  • A. fumigatus
  • A. flavus
  • A. niger
  • A. terreus
<1 CFU per gram
Total Yeast Mold < 100,000 CFU per gram
<10 CFU per gram in Non-Oral Transmucosal Products only
Staphylococcus Aureus <1 CFU per gram in Non-Oral Transmucosal Products only
Bile Tolerant Gram Negative Bacteria <1 CFU per gram in Non-Oral Transmucosal Products only
Total Aerobic <100 CFU per gram in Non-Oral Transmucosal Products only
Georgia
View Regulations

Not Legal
Medical use and recreational use is not permitted
except for CBD oil for medical use.
Guam
View Regulations

Medical Legalized
Medical use is permitted but there are no regulations at this time
Hawaii
View Regulations

Medical Legalized
Unprocessed and processed materials
Salmonella Not detected in 1 gram
E.Coli Not detected in 1 gram
Total Aerobic <100,000 CFU per gram
Total Yeast Mold <20,000 CFU per gram
Bile Tolerant Gram Negative Bacteria <1,000 CFU per gram
Total Coliform <1,000 CFU per gram
Aspergillus

  • A. fumigatus
  • A. flavus
  • A. niger
<1 CFU per gram
Carbon dioxide and solvent based extracts
Salmonella Not detected in 1 gram
E.Coli Not detected in 1 gram
Total Aerobic <10,000 CFU per gram
Total Yeast Mold <100 CFU per gram
Bile Tolerant Gram Negative <100 CFU per gram
Total Coliform <100 CFU per gram
Aspergillus

  • A. fumigatus
  • A. flavus
  • A. niger
<1 CFU per gram
Illinois
View Regulations

Medical + Recreational
Legalized
Salmonella Not detected in 1 gram
STEC E.Coli Not detected in 1 gram
Total Aerobic <104 CFU per gram
Total Yeast Mold <103 CFU per gram
Bile Tolerant Gram Negative Bacteria <102 CFU per gram
Total Coliform <102 CFU per gram
Idaho
View Regulations

Not Legal
No testing regulations
Indiana
View Regulations

Not Legal
No testing regulations
Iowa
View Regulations

Medical Legalized
Consumable Products
Salmonella Not detected in 1 gram
STEC E.Coli Not detected in 1 gram
Total Aerobic <103  CFU per gram
Total Yeast Mold <102  CFU per gram
Inhalable Products
Salmonella Not detected in 1 gram
STEC E.Coli Not detected in 1 gram
Total Aerobic <102  CFU per gram
Total Yeast Mold <101  CFU per gram
Aspergillus

  • A. fumigatus
  • A. flavus
  • A. niger
  • A. terreus
<102  CFU per gram
Non-consumable Products (Topical, Suppositories)
Total Aerobic <103  CFU per gram
Total Yeast Mold <102  CFU per gram
Kansas
View Regulations

Not Legal
No testing regulations
Kentucky
View Regulations

Not Legal
No testing regulations
Louisiana
View Regulations

Medical Legalized
Salmonella <1 CFU/gram
STEC E.Coli <1 CFU/gram
Total Aerobic <10,000 CFU/gram
Maine
View Regulations

Medical + Recreational
Legalized
Unprocessed Materials and Processed Materials
Salmonella Not detected in 1 gram
STEC E.Coli Not detected in 1 gram
Total Aerobic <105 CFU per gram
Total Yeast Mold <104 CFU per gram
Bile Tolerant Gram Negative Bacteria <103 CFU per gram
Total Coliform <103 CFU per gram
CO2 and Solvent Based Extracts
Salmonella Not detected in 1 gram
STEC E.Coli Not detected in 1 gram
Total Aerobic <104 CFU per gram
Total Yeast Mold <103 CFU per gram
Bile Tolerant Gram Negative <102 CFU per gram
Total Coliform <102 CFU per gram
Maryland
View Regulations

Medical Legalized
Salmonella Not detected in 1g
E.Coli <100 CFU per gram
Total Aerobic <100,000 CFU per gram
Total Yeast Mold <10,000 CFU per gram
Massachusetts
View Regulations

Medical + Recreational
Legalized
Salmonella Not Detected in 1 g
STEC E.Coli Not Detected in 1 g
Total Aerobic < 105 CFU/g
Total Yeast Mold < 104 CFU/g
Bile Tolerant Gram Negative Bacteria < 103 CFU/g
Total Coliform < 103 CFU/g
Michigan
View Regulations

Medical + Recreational
Legalized
Bud, shake, trim products
Salmonella Not Detected in 1 g
STEC E.Coli Not Detected in 1 g
Total Yeast Mold <10,000 CFU/g
Total Coliform <1,000 CFU/g
Aspergillus

  • A. fumigatus
  • A. flavus
  • A. niger
  • A. terreus
If Aspergillus spp. is detected, the sample fails
Infused Products
Salmonella Not Detected in 1 g
STEC E.Coli Not Detected in 1 g
Total Yeast Mold <10,000 CFU/g
Total Coliform <100 CFU/g
Aspergillus

  • A. fumigatus
  • A. flavus
  • A. niger
  • A. terreus
If Aspergillus spp. is detected, the sample fails
CO2 and Solvent based extracts
Salmonella Not Detected in 1 g
STEC E.Coli Not Detected in 1 g
Total Yeast Mold <1,000 CFU/g
Total Coliform <100 CFU/g
Aspergillus

  • A. fumigatus
  • A. flavus
  • A. niger
  • A. terreus
If Aspergillus spp. is detected, the sample fails
Minnesota
View Regulations

Medical Legalized
Salmonella Not detected in 1 gram
E.Coli <7 CFU/g
Total Aerobic <100,000 CFU/g
Total Yeast Mold <1,000 CFU/g
Bile Tolerant Gram Negative Bacteria <150 CFU/g
Mississippi
View Regulations

Not Legal
No testing regulations
Missouri
View Regulations

Medical Legalized
Salmonella Not Detected in 1 g
STEC E.Coli Not Detected in 1 g
Aspergillus spp

  • A. fumigatus
  • A. flavus
  • A. niger
  • A. terreus
Not Detected in 1 g
Montana
View Regulations

Medical Legalized
Salmonella Not Detected in 1 g
E.Coli Not Detected in 1 g
Total Yeast Mold <10,000 CFU/g
Nebraska
View Regulations

Not Legal
No testing regulations
Nevada
View Regulations

Medical + Recreational
Legalized
Usable marijuana and crude collected resins, as received, wet marijuana
Salmonella Not Detected in 1 g
STEC E.Coli Not Detected in 1 g
Total Enterobacteriaceae < 1,000 colony forming units per gram
Total Coliform < 1,000 colony forming units per gram
Aspergillus

  • A. fumigatus
  • A. flavus
  • A. niger
  • A. terreus
Not Detected in 1 g
Total Yeast Mold < 10,000 colony forming units per gram
Extract of marijuana (nonsolvent) like hashish, bubble hash, infused dairy butter, mixtures of extracted products or oils or fats derived from natural sources, including concentrated marijuana extracted with CO2, Extract of marijuana (solvent-based) made with any approved solvent, including concentrated marijuana extracted by means other than with CO2
Salmonella Not Detected in 1 g
STEC E.Coli Not Detected in 1 g
Total Enterobacteriaceae < 1,000 colony forming units per gram
Aspergillus

  • A. fumigatus
  • A. flavus
  • A. niger
  • A. terreus
Not Detected in 1 g
Total Yeast Mold < 10,000 colony forming units per gram
Edible marijuana products and Liquid marijuana products, including, without limitation, soda or tonic, including a product which contains concentrated marijuana
Total Enterobacteriaceae < 1,000 colony forming units per gram
Salmonella Not Detected in 1 g
STEC E. coli Not Detected in 1 g
Total aerobic count < 100,000 colony forming units per gram
New Hampshire
View Regulations

Medical Legalized
Harvested Cannabis
STEC E.Coli Not Detected in 1 g
Total Aerobic < 105 CFU/g
Total Yeast Mold < 104 CFU/g
Bile Tolerant Gram Negative Bacteria < 103 CFU/g
Total Coliform < 103 CFU/g
Cannabis Concentrate
Salmonella Not Detected in 1 g
Total Aerobic < 104 CFU/g
Total Yeast Mold < 103 CFU/g
Bile Tolerant Gram Negative < 102 CFU/g
Total Coliform < 102 CFU/g
New Jersey
View Regulations

Medical Legalized
No testing regulations
New Mexico
View Regulations

Medical Legalized
Salmonella Absent in 10 grams cfu/g or cfu/mL
E.Coli <1000 cfu/g or cfu/mL
Total Aerobic <100,000 cfu/g or cfu/mL
Total Yeast Mold <1000 cfu/g or cfu/mL
Bile Tolerant Gram Negative Bacteria <1000 cfu/g or cfu/mL
Total Coliform <1000 cfu/g or cfu/mL
New York
View Regulations

Medical Legalized
Salmonella
E.Coli
Bile Tolerant Gram Negative Bacteria
Pseudomonas (Vaporized products)
Total Enterobacteriaceae
North Carolina
View Regulations

Not Legal
No testing regulations
North Dakota
View Regulations

Medical Legalized
Dried Leaves or Flowers and Medical Cannabinoid Products
Salmonella Not detected in one gram
STEC E.Coli Not detected in one gram
Total Aerobic 105 CFU/g
Total Yeast Mold 104 CFU/g
Bile Tolerant Gram Negative Bacteria 103 CFU/g
Total Coliform 103 CFU/g
Cannabis Concentrates
Salmonella Not detected in one gram
STEC E.Coli Not detected in one gram
Total Aerobic 104 CFU/g
Total Yeast Mold 103 CFU/g
Bile Tolerant Gram Negative Bacteria 102 CFU/g
Total Coliform 102 CFU/g
Ohio
View Regulations

Medical Legalized
Salmonella
STEC E.Coli
Total Aerobic
Total Yeast Mold
Bile Tolerant Gram Negative Bacteria
Total Coliform
Oklahoma
View Regulations

Medical Legalized
Salmonella <1 CFU per gram
E.Coli <1 CFU per gram
Staphylogcoccus aureus <1 CFU per gram
Total Yeast Mold <104 CFU per gram
Oregon
View Regulations

Medical + Recreational
Legalized
Salmonella Not Detected
E.Coli <1 CFU per gram
Pennsylvania
View Regulations

Medical Legalized
Salmonella Not detected in one gram
E.Coli Not detected in one gram
Total Aerobic <10,000 CFU/g Harvest Lot
<10,000 CFU/g Process Lot (including flower at product testing)
Total Yeast Mold <10,000 CFU/g Harvest Lot
< 1,000 CFU/g Process Lot
< 10,000 CFU/g Final processed flower at product testing)
Bile Tolerant Gram Negative <1,000 CFU/g Harvest Lot
<100 CFU/g Process Lot
<1,000 CFU/g Final flower at product testing)
Puerto Rico
View Regulations

Medical Legalized
No testing regulations
Rhode Island
View Regulations

Medical Legalized
Salmonella Not detected in one gram
STEC E.Coli Not detected in one gram
Total Aerobic <100,000 CFU/g
Total Yeast Mold <10,000 CFU/g
Bile Tolerant Gram Negative Bacteria <1,000 CFU/g
Total Coliform <1,000 CFU/g
South Carolina
View Regulations

Not Legal
No testing regulations
South Dakota
View Regulations

Not Legal
No testing regulations
Tennesse
View RegulationsNot Legal
No testing regulations
Texas
View Regulations

Medical use of CBD oil
No testing regulations
Utah
View Regulations
USP Regulations

Medical Legalized
Total Aerobic <105 CFU per gram
Total Yeast Mold <104 CFU per gram
Bile Tolerant Gram Negative <103 CFU per gram
Salmonella Absent in 10 grams
E.Coli Absent in 10 grams
Vermont
View Regulations

Medical + Recreational
Legalized
Salmonella < 200 Colony Forming Unit CFU/g
STEC E.Coli < 200 Colony Forming Unit CFU/g
Aspergillus spp

  • A. fumigatus
  • A. flavus
  • A. niger
< 200 Colony Forming Unit CFU/g
Virginia
View Regulations

Not Legal
No testing regulations
Washington
View Regulations

Medical + Recreational
Legalized
Unprocessed Plant Material
Salmonella Not detected in one gram
STEC E.Coli Not detected in one gram
Bile Tolerant Gram Negative Bacteria <104 Colony Forming Unit CFU/g
Extracted or processed Botanical Product
Salmonella Not detected in one gram
STEC E.Coli Not detected in one gram
Bile Tolerant Gram Negative Bacteria <103 Colony Forming Unit CFU/g
West Virgnia
View Regulations

Medical Legalized
Total Aerobic
Total Yeast Mold
Pseudomonas
Aspergillus
Wisconsin
View Regulations

Not Legal
No testing regulations
Wyoming
View Regulations

Not Legal
No testing regulations