30 live organisms were ordered from ATCC and tested for inclusion and exclusion testing on our TYM and TAC. All bacteria are positive for TAC and negative for TYM.

30 live organisms (fungal) were tested for reactivity with TAC primers with 40 cycles of PCR. Zero Fungi amplified.

Limit Of Detection (LOD)

E.coli ATCC# 8739D5- No Matrix

Klebsiella pneumoniae ATCC#700721D- No Matrix

Aeromonas hydrophila ATCC#7966D-5- No Matrix

Enterobacter aerogenes ATCC# 15038D-5 -No Matrix

E.aerogenes ATCC# 15038D-5 LOD in Cannabis Matrix

HEX (Green) signal is Cannabis gDNA Internal Control

Klebsiella is found on Cannabis (Thompson et al) https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(16)30605-X/fulltext

CFU calibrations should be performed on organisms known to colonize cannabis in the clinical literature. Note C.albicans (often used as a matrix control) has never been discovered on cannabis. Thompson et al.

CFU to Ct Calibration on Klebsiella oxytoca ATCC#51983 without matrix interference

1:1,000 and 1:10,000 Dilution of ATCC stock grown 16hrs at 37C
1:100,000 dilution on Klebsiella
1:1 Million dilution Klebsiella