New Testing Solutions for Aspergillus and THCA Synthase

Medicinal Genomics is excited to announce the addition of two new cannabis testing solutions for pathogenic Aspergillus species (qPCR) and THCA Synthase (youPCR).

Aspergillus Multiplex Detection Assay

 

This latest addition to our PathogINDICAtor® Microbial Detection Platform, allows laboratories to test cannabis samples for four pathogenic species of Aspergillus (A. fumigatus, A. flavus, A. niger, and A. terreus) in a single well.

This not only reduces time and labor for cannabis testing laboratories, it also frees up additional wells so labs can process more samples on each instrument run. For example, California labs can test one cannabis sample for all six required microbial targets, using just two wells! The Aspergillus Multiplex in one well and the STEC/Salmonella Multiplex in the other.

We designed the Aspergillus Multiplex assay so that all four species are on the same channel (FAM). That means detection of any of the four pathogenic Aspergillus species will trigger a curve on the FAM channel. We will continue to offer individual Aspergillus assays in our webstore, so labs can run a subsequent test to determine which species caused the positive result.

The Aspergillus Multiplex Detection Assay and Positive Control are both available for purchase on the Medicinal Genomics webstore.

youPCR THCA Marker Assay

The youPCR THCA Assay functions much like our current CBDA detection assay. Using a simple leaf punch, the test can detect whether the THCA synthase gene is present. A positive result will cause the pink testing solution to turn yellow, which means the plant has either one or two copies of the active THCA allele. That means the plant is either a Type I (all THCA) or Type II plant (50:50 CBDA:THCA).

When the THCA Marker Assay is used in conjunction with the CBDA Marker Assay you will be able to determine whether the plant is a Type I, Type II, Type III (All CBDA), or Type IV (CBGA). See the image below.

Breeders can use this information to get early feedback on their breeding efforts and identify the plants they want to breed within to produce new cannabinoid profiles.

While the plant’s genetics can be coached to express varying concentrations of cannabinoids, the relative ratios of THCA and CBDA are usually genetically determined. As an example, there are no known agricultural methods to make a CBDA-dominant strain become a THCA-dominant strain via environmental conditions. These critical chemotypes are governed by gene knock outs in their respective enzymatic synthases (CDBA Synthase and THCA Synthase). It is those genes that we target with our youPCR tests.

The THCA Marker Assay and Positive Control are both available for purchase on the Medicinal Genomics webstore.