Tobacco Mosaic Virus in Cannabis

Tobacco Mosaic Virus (TMV) is a positive-sense single-stranded RNA virus that infects a number of different plants, most notably tobacco. TMV was identified in 1930, making it the first virus ever discovered. Scientists determined the virus was responsible for the infectious disease in tobacco plants after they observed its ability to reproduce and multiply inside tobacco cells.  

How does Tobacco Mosaic Virus affect Cannabis and Hemp Plants?

Although there are no cases of TMV infecting cannabis or hemp plants in the literature, many growers suspect TMV in plants that develop a mosaic-like pattern of discoloration on the leaves that cannot be attributed to nutrient deficiencies. Our team has screened several suspected TMV plants only to find Hop Latent Viroid. While this does not rule out the possibility of TMV infecting cannabis and hemp plants, we have not been able to confirm an infection, yet. Perhaps you will be the first!

Tobacco mosaic virus symptoms observed in other plants include mosaic, mottling leaves, necrosis, stunting, leaf curling, and yellowing of plant tissues. In fruiting plants, TMV infection can also cause reduced yield and poor fruit quality.  

How does Tobacco Mosaic Virus spread?

TMV spreads primarily via direct contact with an infected plant. Plants that rub against each other transmit TMV, as well as hands that have touched an infected plant. It is also possible that people who consume tobacco products can spread the virus to plants. TMV is exceptionally stable, and purified virus has been reported to be infectious after 50 years storage in the laboratory at 4°C.  

How can Tobacco Mosaic Virus be Controlled?

Growers typically remove any plants infected with TMV because there is no cure. Growers should screen mother plants with qPCR tests to make sure any cuttings that are taken will be virus-free. Cultivators can also screen incoming clones with qPCR assays to make sure they are not introducing infected plants to their grow. 

Test for Tobacco Mosaic Virus with PathoSEEK

Medicinal Genomics offers a variety of PathoSEEK qPCR assays for plant pathogens, including Tobacco Mosaic Virus. DNA can be extracted from infected fan leaves using the Leaf Punch Lysis, and the detection assay can be run on the Agilent AriaMX and BioRad CFX96. 

Available Lettuce Chlorosis Virus Products

Item # Name Reactions
420128 PathoSEEK® Tobacco Mosaic Virus Detection Assay 200 Buy Now
420132 PathoSEEK® Tobacco Mosaic Virus Positive Control 50 Buy Now

Prefer to Outsource Testing?

Cultivators who don’t have enough volume, lab experience, or start-up cash to test plants in-house can outsource to one of our partner labs. Fill out the form below and we will connect you with one in your area, if available.


Available Total Aerobic Count Products

Item # Name Reactions
420106 PathoSEEK™ Total Aerobic Count Detection Assay 200 Buy Now
420314 PathoSEEK™ Total Aerobic Count Positive Control 60 Buy Now